We can perform sequencing on different types of sample including:
- Proteins in solution
- Gel bands
- PVDF blot
Blots must be sent on a PVDF membrane only. However we are also able to transfer samples should this be required. If you have requirements outside our standard specification, or any other queries, please contact us to discuss your needs.
Please note there is a requirement of a minimum of five sequencing cycles.
Specific amino acids
Cysteine: Cysteine residues require modification to a stable derivative before sequencing. Please inform us if you require positive identification of cysteine.
Proline: Where possible, information regarding the position of any proline residues is desirable if a long read is required, as they can cause read overlaps which interfere with accurate sequencing. AltaBioscience has a protocol to reduce the overlap possibility to improve sequencing reads.
Staining of samples:
We are unable to provide sequencing for silver or gold stained samples. Widely available and compatible stains include:
- Ponceau S
- Amido black
- Coomassie, or equivalent
For your convenience, please complete our Protein sequencing sample submission and include it when sending your samples.
Please send samples for protein sequencing to:
Protein sequencing section,
Alta Bioscience Ltd,
37, Walkers Road
B98 9HE, UK
Samples can be posted by ordinary mail but the use of padded bags is highly recommended as envelopes tend to tear in the sorting machines. Blots, gel slices and beads are usually very stable during shipping, so there is no need to freeze the samples. Please do not send whole gels by post.
For samples shipped from outside the UK, please mark shipments as “Samples for Analysis” and include a nominal value <£10 in total on any paperwork where applicable.
This case study highlights the work performed by our scientists to overcome the challenges in the development of this adapted amino acid analysis method.
Amino acid analysis methods review: Post column detection method compared to Kjeldahl and Dumas methods
We discuss three methods widely used for the acquisition of protein content in food products. Methods are briefly explained and the advantages and disadvantages of each explored.
Alta Bioscience Ltd, a University of Birmingham spinout company operating in the areas of custom peptide synthesis and amino acid analysis, announces the appointment of Dr Siobhan Daniels to the Board of Directors with immediate effect.