For more than 20 years AltaBioscience have been supplying custom DNA oligos of outstanding quality for various applications in a ready-to-use form. We offer a full range of modifications and fluorescently tagged primers.
DNA Oligo Synthesis
We pride ourselves on a fast turnaround with next day dispatch.
For routine PCR and sequencing applications, we offer a range of scales and purity options.
- 0.05umole, small scale (default)
- 0.2umole, standard scale (default)
- 1.0umole, large scale
- 15umole, large scale
For all oligos incorporating modifications and special bases, the minimum synthesis scale is 0.2umole.
*Please note that these are starting scales. Your final product may contain less than that amount due to synthesis and purification losses.
| Purification method | Description | Application |
| Synthesis grade (no additional purification) | Synthesis grade oligos are suitable for use in routine PCR and sequencing applications. | Recommended for routine PCR and sequencing applications. |
| Desalting (gel filtration chromatography) | Any residual salts are removed by gel filtration chromatography. Desalt is an essential minimum for mutagenesis work, and recommended for AFLP and microarrays. | Recommended for mutagenesis work. |
| Reverse phase cartridge purification | This is the most popular method for routine removal of any truncated sequences. | Recommended for cloning and insertion. |
| Reverse phase HPLC | Reverse phase HPLC purification producing high purity oligos, with chromatograms provided for QC purposes. HPLC is ideal for larger synthesis scales up to multiple milligrams of oligo yield. | Recommended for high purity modified oligos, e.g. molecular probes and large scale oligos. |