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PCRD Nucleic Acid Detection Kits

A Rapid Alternative to DNA Agarose Gel Electrophoresis

AltaBioscience supplies nucleic acid lateral flow immunoassays (NALFIA), called PCRD, which can be used as an alternative to using DNA agarose gel electrophoresis. Taking 10 minutes or less to detect the successful amplification of target DNA sequences, PCRD is the choice for busy laboratories requiring fast results for DNA amplification exercises.

What is PCRD Nucleic Acid Detection?

PCRD Nucleic acid lateral flow assays are qualitative rapid assays designed to detect amplicons labelled with DIG/Biotin and/or FITC (or FAM)/Biotin, with the addition of a control line.

Detection of Amplified DNA Products using Lateral Flow Assays

PCRD is a rapid, safe and sensitive way to confirm the successful outcome of a DNA amplification exercise. The lateral flow test strips incorporate reagents which sequentially capture and allow the visualisation of double-stranded amplification products containing selected binding partners.

This simple method of amplicon detection takes only 10 minutes, and the result is detectable by eye due to an aggregation of carbon particles at the capture lines.

Also, PCRD is a rapid, safe and sensitive alternative to ethidium bromide staining of agarose gels

Advantages of using PCRD

In addition to being able to cope with large workloads, owing to their ease of use, the following benefits make PCRD an evident choice for post DNA amplification nucleic acid confirmation.

  • Safe to use – avoids use of intercalating dye and UV light
  • No special chemistry or equipment required
  • Time to result of 10 minutes
  • Easy to use
  • High sensitivity
  • Many strips can be run simultaneously
  • Cost effective

Compatible Amplification Methods

PCRD assays can be used in conjunction with the following amplification methods*:

  • Polymerase Chain Reaction (PCR)
  • Loop mediated isothermal amplification (LAMP)
  • Recombinase Polymerase Amplification (RPA)
  • Helicase dependent amplification (HDA).

*Please note that operating protocols may differ between each method.

Applications

 

Due to their versatility, our nucleic acid detection assays can be used in a number of applications. Examples include:

  • Meat speciation testing
  • Food and drink authenticity
  • Rapid disease detection – veterinary, human and plant amongst others

 Oligo synthesis services

For more than  30 years AltaBioscience have been supplying custom  DNA oligos of outstanding quality for various applications in a ready-to-use form. We offer a full range of modifications and fluorescently tagged primers.

We pride ourselves on a fast turnaround with next day dispatch for routine PCR and sequencing applications, we offer a range of scales and purity options.

We can provide primer sequences tagged with Biotin/FITC and FAM for use in our PCRD kits.

For more information, visit our ‘DNA Oligo Synthesis’ page

PCRD Kit Contents and Price                             Product code: AB-PCRD

  • 50 x PCRD Nucleic Acid Detection lateral flow assays
  • 5 x Extraction Buffer (10 mL)
  • Instructions for use

 

For more information, please email us at info@altabioscience.com or call us on +44(0)121 414 5450 to speak to one of our experts.