AltaBioscience has provided custom synthesis of histone peptides from human H2a, H2b, H3 and H4 regions for more than 18 years, having collaborated with the original scientist who developed the idea for histone assays.
Each set contains peptides with modifications such as methyl lysine, dimethyl lysine, acetyl lysine, phosphorylated amino acids and citrulline. In addition, we supply biotinylated histone peptides, including those in Histone Set 5 Sequences.
Sets and histone arrays held in stock and available to ship contain:
- Peptides from Histone H1, Histone H2A, Histone H2B, Histone H3 and Histone H4
- Post-translational modifications included in our sets and histone arrays providing hundreds of different combinations
- Biotinylated histone peptides with spacers incorporated providing more effective screening for epigenetic studies
These are available as individual purified peptides or as sets in the following formats:
- Unbound peptides in deep well trays
- Peptides immobilised on microtitre plates (ELISA format)
- Peptides immobilised on microarray slides
All individual histone peptides are supplied purified to >90%. Information of the stock held and sequence can be found here. Our peptide stock includes H3K9acK14ac, H3K27me1, H4K5acK12ac biotin and many other modified peptides.
If you don’t see the sequence you require from our stock list, or if you would like to add a specific histone peptide to our stock, please contact us and we will synthesise it for you.
Our histone peptide microarrays are ideal for assay development, regulatory studies and assessing the quality of antibodies used in reporter systems. They are also powerful tools for epigenetic studies including:
- binding and pull-down studies
- protein-protein interactions
- enzyme affinity studies
- antibody screening assays
Each peptide can include post-translational modifications including acetylation, methylation, phosphorylation and citrullination.
What are histones?
Histones are the primary protein components of chromatin, the complex of DNA and protein found in eukaryotic cells. They are positively charged which allows them to associate with DNA, which is negatively charged.
Histones and their variants are responsible for organising the chromatin complex and are classified into core histones (H2A/H2B, H3/H4) and linker histones (histones of the H1/H5 family).
Linker histones, H1/H5, bind to nucleosomes to effect folding and compaction of the nucleosomes. H1 and its homologous protein H5 are involved in higher-order structures of chromatin whereas the core histones associate with DNA to form nucleosomes acting as spools around which DNA winds. H1 (or H5) has about 220 residues and the core histones are smaller, each consisting of 100-150 residues.
The five primary families of histones are H1/H5, H2A, H2B, H3 and H4.
Histone peptide sets and stock histone peptides
AltaBioscience has a selection of individual histone peptides and 5 different ready-to-use sets available for purchase.
Use the following links to view our range of stock peptides currently available:
For stock availability of individual peptides or sets, or to discuss custom synthesis requirements, please contact us at firstname.lastname@example.org or call +44(0)1527 584495 to speak to one of our peptide chemists.
Protein and peptide (epitope) tag sequences are attached to proteins to facilitate easy detection and purification of expressed proteins.
In this article, we discuss methods for generating custom antibodies using peptide antigens including peptide antigen design considerations and options for generating antigenic peptides. In general, synthetic peptides are too small to elicit an immunogenic response.
This article is the 3rd in a series on the synthesis of peptides. It looks at solubilising peptides and peptide storage. Peptides are supplied as lyophilised materials, which are regarded as stable compounds for shipping purposes.
Our instrumentation allows for single batch synthesis of up to 576 small scale peptides in one operation, as well as individual peptides in multigram amounts.
In this article we detail some of the design considerations we use in peptides synthesis. This article is available to view here.