Peptide libraries and arrays
AltaBioscience has expertise in synthesising 3 types of peptide library which include peptide mixtures, Episcan peptide libraries and peptides immobilised on slides. Each type has been developed to enable testing and scanning of multiple peptides for fast efficient low cost studies. Studies where these products have been successfully applied included epigenetic studies, protein-peptide interactions and histone peptide applications.
Peptide library options
A single peptide made with specified positions having degenerate sites or mixtures of amino acids. For example, the peptide sequence LEYASXFE where X can be a mixture of several amino acids e.g. Ser, phosSer, Gly.
Large numbers of individual peptides
Up to 576 peptides can be produced in one synthesis batch using the AltaBioscience Episcan technology. Ideal for epitope scanning, Alta Bioscience designed and built the EpiScan II instrumentation to enable the synthesis of large numbers of peptide arrays / libraries / sets, at very low cost.
Primarily intended for screening and epitope scanning.
- From 48 to 576 peptides produced in one synthesis batch, freeze dried, ready for use
- Supplied in 8 x 12 microtitre tray format, deep well microtitre trays or individually numbered 1.2 ml tubes
- Both C-terminal acids or amides in any synthesis set
- N-terminal acetylation, biotinylation dyes etc
- Unusual amino acids can be incorporated
- QC by mass spectrometry
- Yields of 1 – 2 mg of each peptide
Directly compatible with our micro array spotting system or any other robotic device
The Episcan software used can handle either individual sequences or scan a protein, automatically generating overlapping peptides.
Peptide microarrays are made by interfacing directly with the high throughput Episcan peptide synthesis system. The peptides are printed onto coated glass slides directly from the microtitre trays used in the synthesis of the peptides.
- Up to 192 different peptides per slide, each peptide printed in duplicate
- Direct copy of the 96 well arrangement on the slide
- Highly specific binding allows precise peptide orientation on the surface
- Use of a spacer between peptide and the binder molecule ensures high peptide availability
- Most of the common peptide modifications can be used in the microarray set
- Slides are compatible with most imaging systems
A range of ready-to-use histone microarrays and kits can be purchased directly from us.
For a bespoke microarray synthesis service or for more information, please email us at firstname.lastname@example.org or call us on +44(0)1527 584495 to speak to one of our experts.
Protein and peptide (epitope) tag sequences are attached to proteins to facilitate easy detection and purification of expressed proteins.
In this article, we discuss methods for generating custom antibodies using peptide antigens including peptide antigen design considerations and options for generating antigenic peptides. In general, synthetic peptides are too small to elicit an immunogenic response.
This article is the 3rd in a series on the synthesis of peptides. It looks at solubilising peptides and peptide storage. Peptides are supplied as lyophilised materials, which are regarded as stable compounds for shipping purposes.
Our instrumentation allows for single batch synthesis of up to 576 small scale peptides in one operation, as well as individual peptides in multigram amounts.
In this article we detail some of the design considerations we use in peptides synthesis. This article is available to view here.