DNA Oligo Synthesis

*Please note that these are starting scales. Your final product may contain less than that amount due to synthesis and purification losses.

Applications for DNA oligo synthesis and DNA primers

DNA oligos and DNA primers are critical components of a wide range of molecular biology applications, from basic research to clinical diagnostics. Oligos are used routinely for:

• PCR primers
• DNA sequencing and barcoding
• Conjugation to a peptide carrier, e.g., to transport a peptide inside the cell nucleus
• Gene detection, synthesis and cloning
• Site-directed mutagenesis
• Fluorescence in situ hybridization (FISH)
• Antisense oligonucleotide therapy
• RNA interference (RNAi)
• Aptamer development
• Microarray analysis
• CRISPR/Cas9 genome editing
• Detection and identification of genetic mutations or polymorphisms
• SNP genotyping (Single Nucleotide Polymorphism)

Custom DNA oligo synthesis method

DNA oligos are synthesised using solid-phase methods. The synthesis is performed using automated synthesisers where reactions proceed rapidly, one base addition occurring every four minutes with very high efficiencies. The chemistry is well established with the base at the 3’ of the target oligo being attached to a controlled pore glass support, with the oligo grown automatically base by base towards the 5’ end.

Degenerate oligos (mixed bases) of any combination can be added at any position at no extra cost. Codes for degenerate bases are as follows:

Unusual bases

Internal modified bases, e.g. Inosine, Uracil, Oxo dG, Bromo dU and Hydroxy dC, can be added anywhere along the chain.

3’ and 5’ modifications

These include groups such as biotin, amino, thiol, phosphate groups or linkers. Incorporation of dyes and quenchers at the 3’ end is available, with options such as BHQ 1, BHQ 2, BHQ 3, Dabcyl and FAM readily available.

Frequently asked questions