Skip to content

DNA Oligo Synthesis and Primers

AltaBioscience has been supplying custom DNA oligos and primers of outstanding quality for various applications in a ready-to-use form for 50 years. Our DNA oligo synthesis service offers a full range of modifications, scales, purification options and fluorescently tagged custom DNA primers.


UK-based ISO 9001-certified laboratory with longstanding expertise of 50 years serving clients globally.


Our DNA oligo synthesis services work within industry-leading turnaround times, with single-stranded DNA (ssDNA) completed and supplied within 7-10 working days.


Our highly skilled specialists can offer you professional assistance throughout the whole project, from your initial enquiry right through to completion.


We synthesise custom single-stranded DNA with a flexible range of scales and purities to match your specifications. We also offer custom peptide synthesis and DNA-peptide synthesis services.


DNA oligos are synthesised using solid phase methods. Synthesis is performed using automated synthesisers where reactions proceed rapidly with very high efficiencies.


We routinely work with pharmaceutical and biotech companies, research institutes, university academics and contract manufacturing companies.

DNA oligo synthesis services

Here at AltaBioscience, our DNA oligo synthesis service offers single-stranded DNA (ssDNA) with a fast turnaround from our ISO 9001-certified laboratory.

Wide range of DNA oligos available including various modifications both at the 5’ and 3’ positions and internal modification in the sequence.
DNA oligo synthesis options are from 0.2 umole starting scale upwards Oligo synthesis scales include 0.2 umole, standard scale (default), 1.0 umole, large scale and 15 umole, extra large scale*
Degenerate oligos (mixed bases) of any combination can be added at any position at no extra cost.
All oligos undergo rigorous quality control testing prior to shipment to ensure that you receive the quality product you expect.
All oligos are sent dry by courier and are stable during shipping.
Several options for the purification of DNA oligo primers including desalt purification (default), HPLC purification or unpurified as required for your application.
Oligos can be immobilised on CPG for conjugation options prior to cleavage.
Each oligo is supplied as dry material in individual tubes with a Certificate of Analysis (CoA) detailing melting temperature (TM), molecular weight (MW) and concentration information for reconstruction.
As specialists in DNA-peptide conjugates, our laboratory can also provide DNA-peptide conjugates made to your specific requirements.

*Please note that these are starting scales. Your final product may contain less than that amount due to synthesis and purification losses.

Applications for DNA oligo synthesis and DNA primers

DNA oligos and DNA primers are critical components of a wide range of molecular biology applications, from basic research to clinical diagnostics. Oligos are used routinely for:

  • PCR primers
  • DNA sequencing and barcoding
  • Conjugation to a peptide carrier, e.g., to transport a peptide inside the cell nucleus
  • Gene detection, synthesis and cloning
  • Site-directed mutagenesis
  • Fluorescence in situ hybridization (FISH)
  • Antisense oligonucleotide therapy
  • RNA interference (RNAi)
  • Aptamer development
  • Microarray analysis
  • CRISPR/Cas9 genome editing
  • Detection and identification of genetic mutations or polymorphisms
  • SNP genotyping (Single Nucleotide Polymorphism)

Custom DNA oligo synthesis method

DNA oligos are synthesised using solid-phase methods. The synthesis is performed using automated synthesisers where reactions proceed rapidly, one base addition occurring every four minutes with very high efficiencies. The chemistry is well established with the base at the 3’ of the target oligo being attached to a controlled pore glass support, with the oligo grown automatically base by base towards the 5’ end.

Degenerate oligos (mixed bases) of any combination can be added at any position at no extra cost. Codes for degenerate bases are as follows:

Unusual bases

Internal modified bases, e.g. Inosine, Uracil, Oxo dG, Bromo dU and Hydroxy dC, can be added anywhere along the chain.

3’ and 5’ modifications

These include groups such as biotin, amino, thiol, phosphate groups or linkers. Incorporation of dyes and quenchers at the 3’ end is available, with options such as BHQ 1, BHQ 2, BHQ 3, Dabcyl and FAM readily available.

Frequently asked questions

Related articles

AltaBioscience Ltd

37 Walkers Road,
Manor Side,
B98 9HE

Telephone: +44 (0)1527 584495

Registered in England and Wales: 37 Walkers Road, Redditch, B98 9HE. Registered Number: 07278564