- Bulky or inhomogenous samples must be blended before hydrolysis. The largest amount of sample that can be hydrolysed is about 200mg.
- At least 20 micrograms of protein are needed for analysis with ninhydrin detection.
- For accurate weighing, >2mg of material must be provided. For special hydrolysis conditions e.g. tryptophan analysis, at least 5mg of material is required.