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The standard inoculation procedure for a polyclonal antibody in a rabbit involves six immunisations at two-week intervals and three bleeds (with an additional pre-immune bleed). Read more about the generation process.
Our monoclonal process is identified by five phases. A typical monoclonal antibody project may yield up to 10 positive wells at the fusion screening stage and, after cloning, can deliver two or three stable monoclonal antibodies. Read more about the process and project steps.
At AltaBioscience, we offer different options for conjugation either at the 3’ end of the oligo or at the 5’ prime end, depending on your requirements. In addition, the linkage between the DNA-peptide can be either a stable covalent bond or a reducible disulphide bridge as needed for your application.
AltaBioscience has provided custom synthesis of histone peptides from human H2a, H2b, H3 and H4 regions for many years, having collaborated with the original scientist who developed the idea for histone assays.
Suitable for both monoclonal antibody production and polyclonal antibody production, our peptide synthesis experts can assist with peptide antigen design using our epitope design software thereby suggesting antigenic peptide regions likely to provide the best immune response.
HPLC is suitable for a wide array of compounds and is routinely used to partition or purify peptides or proteins due to its high resolving power, robustness and extreme reproducibility.
Mass spectrometry analysis detects and quantifies known and unknown materials, mixtures and chemical structures of different molecules to provide precise, quantitative analysis and detection down to ultra-trace levels.
Our method of post-column detection allows us accurately quantify very low levels of phenylalanine in food, drinks and medicinal products for those requiring a low protein diet.
We use the most robust amino acid analysis method available: separation of a mixture of amino acids using ion exchange chromatography followed by post-column photometric detection.
For the analysis of these specific amino acids in samples, AltaBioscience uses different methods that enable us to quantify them accurately.